期刊论文

中文

华北农学报

1000-7091

2022

37

3

19-26

10.7668/hbnxb.20192894

2020NK2023:湖南省重点研发计划项目 2018JJ3233:湖南省自然科学基金

本校为第一机构

为明确陆地棉细胞分裂素受体基因(GhCRE1)在调控植物生长发育中的功能,为研究陆地棉GhCRE1基因调控种子萌发的分子机理提供材料,进一步从分子水平上提高种子萌发率,通过克隆陆地棉GhCRE1基因,利用基因过表达技术将该克隆片段连接至含Ubiquitin启动子的pCUbi1390载体上构建该基因的过表达载体,同时利用CRISPR/Cas9基因编辑技术设计拟南芥CRE1基因的sgRNA片段,合成sgRNA核苷酸序列,从而构建拟南芥CRE1基因靶向敲除载体。将以上2种载体转化农杆菌,使用花序浸染法,以拟南芥为受体材料创建GhCRE1过表达株系及基因编辑功能缺失突变体。接着利用PCR技术和qPCR技术筛选鉴定获得过量表达的GhCRE1拟南芥株...

To clarify the function of the receptor gene for Gossypium hirsutum L.cytokinin receptor(GhCRE1)in the regulation of plant growth and development,and to provide material for studying the molecular mechanism of the GhCRE1 gene in regulating seed germination in Gossypium hirsutum,ultimately improving seed germination rates at the molecular level.The GhCRE1 gene was cloned and the fragment was ligated to the pCUbi1390 vector containing the Ubiquitin promoter using gene overexpression technology to construct GhCRE1 overexpression vector,while the sgRNA fragment of the Arabidopsis CRE1 gene was des...