期刊论文

中文

生物技术

1004-311X

2011

21

2

67-69

10.3969/j.issn.1004-311X.2011.02.052

湖南农大稳定人才基金项目（09WD34）资助；

本校为第一机构

目的:比较紫外线、微波单因子对金针菇J05Y-C单细胞悬液的诱变效应,并筛选获得优势突变菌株.方法:分别采用不同强度的紫外线、微波单因子对金针菇单细胞悬液进行诱变处理,以出发菌株作为对照,比较诱变致死率、正变率、HC值、菌丝生长量,进行优势菌株筛选、传代稳定性试验.结果:诱变效应的最佳剂量范围在致死率75%～90%的处理组;相同处理时间内,微波诱变的正变率大于紫外诱变的正变率,分别为85.00%和76.67%.采用最大功率700W、脉冲频率2540MHz的微波炉,以中等功率强度处理80s,获得HC值、菌丝生长量比出发菌株提高32.38%、62.16%的优势突变株W8011,连续8次传代遗传性能稳定.结论:在试验的金针菇菌...

Objective：The mutagenic effects of UV and microwave on Flammulia velutipes J05Y-c were compared,and high mutant would be obtained.Method：Original strain was mutated by UV and microwave;death rate,forward rate,HC,growth rate of mycelium were compared between original and mutant strain;and genetic stability of high mutant was determined.Result： Optimal mutagenic effects were obtained in death rate 75%-90%;the forward rate of microwave treated was higher than that UV treated at the same given time,85.00% and 76.67% respectively;Optimal microwav...