Metallothionein(MT) extracted from swine liver was linked to bovine serum albumin (BSA) and then immunized to Kunming mice for acquiring the antibody toward MT. An indirect competitive ELISA was established based on acquired antibody and standard MT. The results showed that the antibody was special to MT. The sensitivity of the constructed ELISA was 4.1 ng/mL. Intra- and inter-assay coefficient of variation were 9. 976% and 14. 385 8% respectively. It proved that the indirect competitive ELISA is sensitive, specific, stable, ...