期刊论文

中文

畜牧兽医学报

0366-6964

2010

41

4

463-468

国家自然科学基金（30771616） 长沙科技计划一般项目（K0902144-21）

本校为第一机构

本研究旨在阐明猬迭宫绦虫湖南分离株线粒体细胞色素c氧化酶第I亚基（cox1）基因部分序列（pcox1）和烟酰胺腺嘌呤二核苷酸（NADH）脱氢酶亚单位l基因（nad1）部分序列（pnad1）的遗传变异情况,并用pcox1和pnad1序列重构猬迭宫绦虫与其它绦虫的种群遗传关系。利用聚合酶链反应（PCR）扩增猬迭宫绦虫的pcox1和pnad1,应用ClustalX1.81程序对序列进行比对,再用Phylip3.67程序MP法和Mage4.0程序NJ法绘制种系发育树,并用Puzzle5.2程序构建最大似然树,同时利用DNAStar5.0中的Megalign程序进行同源性分析。结果显示所获得各样品株的pcox1和pnad1序列长度一致,分别为396和566bp,湖南分离株与已知猬迭宫绦...

The objectives of the present study were to examine sequence variation in the mitochondrial cytochrome coxidase subunit 1（cox1）gene and NADH dehydrogenase subunit 1（nad1）gene among Spirometra erinaceieuropaei isolates from Hunan Province,and to reconstruct their phylogenetic relationship using cox1 and nad1 sequences.The partial cox1（pcox1）and nad1（pnad1）was amplified from each S.erinaceieuropaei sample,and pcox1 and pnad1 sequences were aligned using the ClustalX 1.81.MP and NJ trees of pcox1 and pnad1 were constructed using the softwa...