期刊论文

中文

畜牧兽医学报

0366-6964

2010

41

12

1592-1597

国家自然科学基金（30771616） 湖南省教育厅重点项目（06A028） 长沙科技计划一般项目（K0902144-21）

本校为其他机构

本研究旨在阐明湖南省鸡蛔虫分离株线粒体细胞色素c氧化酶第I亚基(cox1)基因部分序列(pcox1)和烟酰胺腺嘌呤二核苷酸(NADH)脱氢酶亚单位l基因(nad1)部分序列(pnad1)的遗传变异情况,并用pcox1和pnad1序列构建鸡蛔虫与其它科蛔虫的种群遗传关系.作者应用聚合酶链反应(PCR)扩增鸡蛔虫虫株的pcox1和pnad1,应用Clustal X1.81程序对序列进行比对,然后用Phylip3.67程序MP法和Mega4.0程序NJ法绘制种系发育树,并用Puzzle5.2程序构建最大似然树.所获得的pcox1和pnad1序列长度均为394和408bp,种系发育树表明,20个分离株鸡蛔虫位于同一分枝.由于鸡蛔虫pcox1和pnad1序列种内相对保守,种间差异较大,故可作为种...

The objectives of the present study were to examine sequence variation in the mitochondrial cytochrome c oxidase subunit 1（cox1） and NADH dehydrogenase subunit 1（nad1） genes among Ascaridia galli isolates from Hunan province,and to study the phylogenetic relationship among roundworms of the other family using cox1 and nad1 sequences.The partial cox1（pcox1） and nad1（pnad1）were amplified from each Ascaridia galli sample,and pcox1 and pnad1 sequences were aligned using the ClustalX 1.81.MP and NJ trees were constructed by using the softwar...