期刊论文

中文

畜牧兽医学报

0366-6964

2012

43

1

57-64

国家自然科学基金项目（30972099） 现代农业产业技术体系项目 湖南省科技计划项目（2009FJ4053） 湖南农业大学人才稳定基金（2007WD03）

本校为第一机构

将90只大鼠随机分为5组,分别注射10μg·100μL~(-1) pcISI(T_1)、50μg?100μL~(-1) pcISI(T_2)、100μg?100μL~(-1)(T_3)pcISI、100μg空载体pcMV-S(V)和100μL生理盐水(S),以探讨在不使用免疫佐剂的情况下,不同剂量的双拷贝抑制素pcISI基因免疫对大鼠卵泡发育、产仔和生殖激素的影响。结果表明,加强免疫能提高抗体P/N值,加强免疫后各剂量组平均抗体P/N值大于2,T_3组抗体P/N值显著高于T_1和T_2组(P

Ninety rats were randomly assigned to five dosage groups of 18 each （T~, T2, T3, V, S, which was immunized with 10, 50, 100 μg · 100 μL^-lof pCISI, 100 μg pcMV-S and 100 μL physiological saline per rat, respectively） to investigate the effect of double copies inhibin pcISI gene immunization on follicle development, production and reproductive hormones without any immune adjuvant. The results showed that the booster immunization could improve P/N value of antibody against inhibin, groups was higher than 2 group was higher than th the aver...