期刊论文

Bacterial Flora Analysis of Saliva and Midgut Contents from Partially or Fully Engorged Female Adult Rhipicephalus microplus

中文

畜牧兽医学报

0366-6964

2017

48

3

530-537

10.11843/j.issn.0366-6964.2017.03.017

国家自然科学基金

本校为第一机构

旨在探明微小牛蜱随吸血时间的延长,中肠和唾液微生物菌群结构的差异,以及优势菌属在中肠和唾液中增殖和迁移的特点。在无菌条件下采集半饱血、饱血微小牛蜱唾液和中肠内容物;提取细菌总DNA,PCR扩增其16SrDNA V3-V4区,Illumina MiSeq双端测序;对序列进行拼接和过滤、OTUs聚类、物种注释及多样性分析。结果显示,共得到134 317条序列,聚类后获得1 304个OTUs,半饱血雌成蜱唾液(SP)、饱血雌成蜱唾液(SF)、半饱血雌成蜱中肠内容物(MP)、饱血雌成蜱中肠内容物(MF)分别获得336、332、269、289个OTUs,其中152个OTUs为4个样本共有;4个样品在门水平上,以变形菌门和厚壁菌门为优势菌门,变形菌门在中肠中的含...

The aim of this study was to investigate the difference of bacterial flora and diversity of the saliva and midgut contents of Rhipicephalus microplus with the extension of feeding time,and the proliferation and migration characteristics of dominant genera in saliva and midgut.The saliva (S) and midgut contents (M) collected from partially (P) or fully (F) engorged adult female ticks under sterile environment,the total DNA of bacteria were extracted and PCR amplification.Using Illumina MiSeq sequencing platform,two-terminal sequencing of V3-V4 areas of 16S rDNA was carried.Then OTUs (operationa...