期刊论文

中文

畜牧兽医学报

0366-6964

2017

48

11

2181-2188

10.11843/j.issn.0366-6964.2017.11.020

国家自然科学基金（31372431） 湖南农业大学东方科技学院青年科学基金（16QNZ13）

本校为第一机构

绵羊虱蝇是绵羊体表常见的吸血寄生虫,对其形态学特征的研究较少。为提供能够快速、准确辨识此寄生虫的依据,本研究通过超景深三维显微系统和扫描电镜对绵羊虱蝇的形态特点进行了观察,并通过分析18S rRNA基因序列进行了分子学鉴定。结果显示,绵羊虱蝇翅完全退化,无平衡棒;头部短小阔扁,无单眼,复眼小,触角极短,刺吸式口器;胸部不分节,有3对足,足末端有发达的爪,4对气门;腹部不分节,雌虫腹部大而圆,末端凹陷;雄虫腹部小,末端凸出;周身布满刚毛。18S rRNA测序结果与GenBank中已公布序列相似度为99.59%。结果表明,本研究从甘肃酒泉绵羊体表采集的寄生虫为绵羊虱蝇,且此寄生虫有较易辨识的形态学特征...

Melophagus ovinus is one of the most common and economically important blood-feed- ing ectoparasites on sheep, but no exhaustive information on the morphology of M. ovinus. The aim of this study was to provide the basis for rapid and accurate identify this parasite. The mor- phological characteristics of M. ovinus were observed by the super depth of field three-dimension- al microscope system and scanning electron microscope, and the molecular identification was also performed by analyzing the gene sequence of 18S rRNA. The results showed that M. ovinus was an entirely wingless brown fly, no h...