期刊论文

Proteomics Analysis of Haemaphysalis flava Eggs

中文

畜牧兽医学报

0366-6964

2019

50

3

627-636

10.11843/j.issn.0366-6964.2019.03.018

湖南省自然科学基金(2018JJ2167) 湖南农业大学“双一流”建设项目(SYL201802016).

本校为第一机构

本研究旨在探明褐黄血蜱卵内蛋白组分,揭示蜱胚胎发育中的关键营养物质,为筛选可干扰胚胎发育的抗原分子奠定基础。采用液相色谱-串联质谱法对新鲜褐黄血蜱的卵蛋白成分进行分析,基于该蜱的唾液腺转录组翻译文库、中肠转录组翻译文库和Uniprot数据库对各蛋白成分进行鉴定。结果显示:从褐黄血蜱卵黄蛋白提取液中检出特异性肽段221条,由此鉴定蛋白53种,其中高可信蛋白12种,能够确定的功能蛋白有肌动蛋白(actin)和卵黄蛋白原Ⅱ(vitellogenin 2,Vg-2),且Vg-2与卵黄蛋白(vitellins,Vn)一级结构相同。蜱卵原生质所含蛋白较少;Vg-2是蜱卵内存在的唯一一种卵黄蛋白原。

The protein components of Haemaphysalis flava eggs were investigated, and the key nutrients were analyzed in the development of embryo, in order to lay a foundation for screening antigen molecules that can interfere with embryonic development. Firstly, the egg protein components of fresh Haemaphysalis flava were analyzed by LC-MS/MS, and based on the salivary gland transcriptome translation library, the midgut transcriptome translation library and the Uniprot database, each protein components were identified. A total of 221 unique peptides were detected from the yolk protein extract of Haemaph...