期刊论文

Effect of Porcine Pseudorabies Virus Infection on Proliferation of PK-15Cell and Expression of Heat Shock Protein 27,70and 90

中文

畜牧兽医学报

0366-6964

2019

50

7

1441-1448

10.11843/j.issn.0366-6964.2019.07.013

31272630:国家自然科学基金 31772819:国家自然科学基金 31572591:国家自然科学基金

本校为第一机构

研究猪伪狂犬病病毒(PRV)-YY株感染对宿主细胞增殖和热休克蛋白(HSP)27、70和90表达的影响。用Reed-Muench法测定PRV-YY滴度后,用不同病毒量感染PK-15细胞,采用iCELLigence实时无标记细胞功能分析仪监测细胞的增殖,并用实时荧光定量PCR(RT-qPCR)和免疫印迹试验(Western blot)分别检测感染后细胞中病毒核酸含量及三种HSPs mRNA和蛋白质表达的变化。结果表明,当用80TCID_(50)和100TCID_(50)的病毒量(滴度为10~6 TCID_(50)·0.1mL~(-1))感染细胞时,在所监测的时间内细胞指数均显著低于对照组;当感染60h时,除10TCID_(50)感染组外,其余各组的细胞指数均极显著低于对照组(P

In present study we investigated the effects of porcine pseudorabies virus(PRV) infection on proliferation and heat shock protein 27,70 and 90 expression of host cells in vitro. PK-15 cells were infected with different amounts of PRV-YY strain after the titer was determined by Reed-Muench method;cell proliferation was monitored by an iCELLigence Real-Time Labeled Cell Function Analyzer after infection;levels of viral nucleic acid and the mRNA and protein expression of HSP27, 70 and 90 in PRV-infected cells were detected using Real-time fluoresc...