期刊论文

Acute Exposure of Bacterial Lipopolysaccharide Induces Testicular Injury in Mice through Inflammatory Response

中文

畜牧兽医学报

0366-6964

2019

50

5

1099-1105

10.11843/j.issn.0366-6964.2019.05.021

31572591:国家自然科学基金 31772819:国家自然科学基金 :国家级大学生创新创业训练计划项目

本校为第一机构

旨在研究细菌脂多糖(LPS)急性暴露对小鼠睾丸及其功能的影响。将健康雄性小鼠随机分为4组:对照组(Control)、1.25mg·kg~(-1) LPS组、2.5mg·kg~(-1) LPS组和5.0mg·kg~(-1) LPS组,对照组腹腔注射200μL生理盐水,其余3组注射等体积含不同剂量的LPS,作用12h。收集睾丸组织制作石蜡切片,染色后观察其病理变化,记录体重、睾丸和附睾指数,并测定精子品质相关指标,用RT-qPCR法检测炎症相关基因TNF-α、IL-6、GAS6和TGF-β1的转录,同时用ELISA检测血清中睾酮(T)的含量。结果表明,与对照组相比,LPS急性暴露时,以剂量依赖性方式降低小鼠体重(P

The present study was conducted to investigate the effects of acute exposure to bacterial lipopolysaccharide (LPS) on testis and its function in mice.Healthy male mice were randomly divided into 4 groups:Control group,1.25 mg·kg^-1 LPS group,2.5 mg·kg^-1 LPS group and 5.0 mg·kg^-1 LPS.The control group was intraperitoneally injected with 200 μL saline,while the other three groups were injected the same volume saline containing different doses of LPS and treated for 12 hours.Testes were collected to check the pathological changes after stain...