期刊论文

Preliminary Identification and Functional Classification of Haemaphysalis hystricis Egg Protein Components

中文

畜牧兽医学报

0366-6964

2021

52

10

2895-2904

10.11843/j.issn.0366-6964.2021.010.020

湖南省重点领域研发计划项目（2019 NK2181）； 湖南农业大学“双一流”建设项目（SYL201802016）；

本校为第一机构

旨在探明豪猪血蜱卵原生质蛋白成分,为探索干扰蜱类胚胎发育和新型控蜱策略奠定基础。采用FASP法消化豪猪血蜱卵蛋白提取物,LC/MS/MS法对其进行分析、检测,搜索褐黄血蜱卵巢转录组文库、唾液腺转录组文库和中肠转录组文库,分别检出特异性肽段359、312、357条,鉴定多肽108、170、161条,终而鉴定高可信蛋白103种。豪猪血蜱蜱卵原生质中富含酶类、蛋白酶抑制剂、转运蛋白、细胞骨架蛋白、蛋白质合成与修饰、分泌蛋白以及未鉴定的蛋白共8类蛋白。

The identification of protein components of Haemaphysalis hystricis egg protoplasm illustrated the tick embryo protein profile and laid a foundation for the discovery of new anti-tick strategies.The egg protein extracts of Haemaphysalis hystricis was digested with trypsin according the FASP procedure and the LC/MS/MS method was employed to detect and identify peptides.The MS data were searched against the ovarian,salivary gland and midgut transcriptomic libraries of Haemaphysalisflava,and the 359,312and 357specific peptides were detected, then ...