期刊论文

The Full-length cDNA Cloning,Sequence Characterization and Expression Pattern Analysis of Porcine ADAR2Gene

中文

畜牧兽医学报

0366-6964

2021

52

03

620-629

10.11843/j.issn.0366-6964.2021.03.006

中国农业科学院科技创新工程（ASTIP-IAS02）； 国家生猪产业技术体系（CARS-36）； 湖南省教育厅科学研究项目（19C0902）；

本校为第一机构

旨在克隆猪作用于RNA的腺苷脱氨酶2基因（ADAR2）全长cDNA序列,同时对该基因在猪不同组织中的表达规律进行探索。利用RACE（rapid-amplification of cDNA ends）对大白猪ADAR2基因mRNA全长序列进行克隆,并进行生物信息学分析;用荧光定量PCR方法检测35日龄大白猪心、肝、肺、肾、脾、脑、小肠、背最长肌和背部脂肪9种组织中ADAR2的表达水平。结果表明,猪ADAR2基因cDNA全长6 305 bp,共包含12个外显子,编码704个氨基酸,与人、黑猩猩、猕猴、长臂猿、黄牛、山羊和绵羊的CDS区核酸序列和氨基酸序列的一致性均在84%以上。该基因编码的蛋白含有2个双链RNA结合基序和一个脱氨酶结构域。猪ADAR2在检测的各...

This study aimed to clone the full-length cDNA of the porcine ADAR2gene and explore its expression pattern in different tissues of pigs.Rapid-amplification of cDNA ends(RACE)was used to clone the full-length cDNA sequence of the ADAR2gene in Large White pigs and the sequence was analyzed by bioinformatics.Real-time PCR was used to detect the ADAR2mRNA expression in the heart,liver,lung,kidney,spleen,brain,small intestine,muscle and backfat of 35-day-old pigs.Porcine ADAR2cDNA sequence of 6 305bp was cloned,which contained 12exons and encoded 704amino acids.The nucleic acid and amino acid seque...