期刊论文

Comparison of Histological Structure and Lactoferrin Expression in Uterine and Ovarian of Dogs during Normal Estrus and with Pyometra

中文

畜牧兽医学报

0366-6964

2021

52

04

1103-1114

10.11843/j.issn.0366-6964.2021.04.026

湖南农业大学2019年度大学生创新创业训练计划项目（xcx19052）； 湖南农业大学2018年青年科学基金项目（18QN24）；

本校为第一机构

本研究旨在探明犬子宫和卵巢在正常发情期与患子宫蓄脓时,其组织结构及乳铁蛋白（lactoferrin, LF）表达的变化特征。应用Masson’s、VVG、PAS组织化学染色方法观察乏情期、发情期、患子宫蓄脓时犬子宫及卵巢的组织结构特点,用免疫组织化学SP法观察LF的分布特征。结果显示,正常发情期犬:乏情期、发情期子宫内膜肌层厚度比分别为0.762 0、0.924 3;乏情期子宫固有层中胶原纤维含量大于发情期,发情期卵巢中胶原纤维含量大于乏情期;子宫血管层及卵巢血管内弹性膜清晰完整;子宫腺在乏情期时浅层管腔小,深层较大、腺管上皮为单层柱状上皮、上皮细胞及管腔内PAS阳性反应较强,发情期子宫腺管腔变大、腺管...

The purpose of this study was to investigate the changes in histological structure and the expression of lactoferrin(LF)in the uterus and ovaries of dogs during the normal estrus and with pyometra.Masson's,VVG and PAS histochemical staining were used to observe the histological characteristics of uteruses and ovaries in anestrus,estrus,and pyometra.The immunohistochemical SP method was used to observe the distribution of LF.The results showed that the endometrium/myometrium ratios in the period of anestrus and estrus were 0.762 0and 0.924 3, respectively.The content of collagen fibers in the u...