期刊论文

中文

畜牧兽医学报

0366-6964

2022

53

3

938-946

10.11843/j.issn.0366-6964.2022.03.026

国家重点研发计划专项（2017YFD0501403）；

本校为第一机构

旨在研究氟马西尼联合肾上腺素对钩吻中毒大鼠的解救效果,并验证钩吻可能的毒性作用靶点,为钩吻中毒机制探讨及解救钩吻中毒病例提供参考。构建钩吻大鼠中毒模型,将60只大鼠随机分为4组,分别为对照组、氟马西尼解救组、肾上腺素解救组、联合用药(氟马西尼+肾上腺素)解救组,记录各组大鼠中毒及死亡情况,并监测各组大鼠血压、心率,评价各组药物的解救效果。采用分子对接、全细胞膜片钳技术,预测钩吻主要毒性物质钩吻素己与解救药物作用的靶点,阐释其可能的解毒机制。结果显示:钩吻灌胃大鼠的半数致死量(LD_(50))为0.25g·kg~(-1),氟马西尼联合肾上腺素使用时对钩吻中毒的大鼠有显著的解救效果,存活...

This study aimed to assessment the detoxification of flumazenil combined with epinephrine on Gelsemium elegans Benth(G.elegans)poisoning rats,providing reference for G.elegans toxic mechanism and poisoning cases.G.elegans poisoning rat model was constructed,and 60 rats were randomly divided into 4 groups:control group,flumazenil rescue group,epinephrine rescue group and combined drug(flumazenil+epinephrine)rescue group.The poisoning and death of the groups’rats were recorded;the blood pressure and heart rate were monitored.Then evaluate the re...