期刊论文

中文

畜牧兽医学报

0366-6964

2022

53

6

1858-1869

10.11843/j.issn.0366-6964.2022.06.019

湖南省自然科学基金（2017JJ2116）；

本校为第一机构

通过对多杀性巴氏杆菌(Pasteurella multocida)CS株全基因组的毒力基因分析,以期从基因组角度了解P.multocida CS的致病性机理。常规方法分离细菌,并进行毒力鉴定。基于二代测序平台对P.multocida CS进行测序,应用比较基因组学方法将其与GenBank中具全基因组来源于不同地域和宿主的P.multocida基因组进行比较分析。结果显示:猪肺疫病料中分离细菌滴鼻感染小鼠,测得其LD_(50)为5×10~2 CFU·mL~(-1),显示较强毒性。将测序后的P.multocida CS株全基因组信息提交至NCBI,获得登录号SUB11119617。通过对P.multocida CS全基因组序列的分析表明,P.multocida CS株全基因组大小为2 599 048bp,G+C含量为39...

This study aimed to investigate the pathogenicity of Pasteurella multocida CS strain by analyzing virulence genes of its complete genome.The bacteria strain was isolated from swine pasteurellosis by conventional methods and its virulence was tested by mice.The complete genome of P.multocida CS strain was sequenced based on the second generation sequencing platform,and analyzed its differences with others submitted to GenBank from different regions and hosts by comparative genomics methods.The LD50 of P.multocida CS strain was 5×102 CFU·mL-1,w...