期刊论文

Studies on the Role of HSP70-b2 and Its 14-Mer-like Peptide from Haemaphysalis flava

中文

畜牧兽医学报

0366-6964

2023

54

06

2570-2580

10.11843/j.issn.0366-6964.2023.06.034

国家自然科学基金青年项目(31902294)； 湖南省自然科学基金青年项目(2020JJ5230)；

本校为第一机构

褐黄血蜱(Haemaphysalis flava,H.flava)热休克蛋白70-b2(heat shock protein 70-b2,HSP70-b2)属于HSP70家族。为评估褐黄血蜱HSP70-b2的抗凝血活性、...展开更多 褐黄血蜱(Haemaphysalis flava,H.flava)热休克蛋白70-b2(heat shock protein 70-b2,HSP70-b2)属于HSP70家族。为评估褐黄血蜱HSP70-b2的抗凝血活性、免疫原性及其类14-Mer肽的作用,本研究通过RACE技术扩增褐黄血蜱HSP70-b 2基因全长;定点突变其编码类14-Mer肽段序列获得HSP70-b2M基因全长;分别构建原核表达质粒,经原核表达、镍柱层析纯化获得可溶性重组蛋白,体外凝血四项试验评估rHSP70-b2与rHSP70-b2M的抗凝血活性;将重组蛋白与弗氏...

Heat shock protein 70-b2(HSP70-b2)of Haemaphysalis flava is a member of the HSP70 family.The research was to evaluate the anticoagulant activity and immunogenicity of HSP70-b2,and to investigate the role of 14-Mer-like peptide of HSP70-b2 from H.flava.The gene of H.flav...MORE Heat shock protein 70-b2(HSP70-b2)of Haemaphysalis flava is a member of the HSP70 family.The research was to evaluate the anticoagulant activity and immunogenicity of HSP70-b2,and to investigate the role of 14-Mer-like peptide of HSP70-b2 from H.flava.The gene of H.flava ...