The present study aimed to develop an indirect ELISA to detect antibodies against the Cap protein of porcine circovirus type 4(PCV4),and to detect PCV4 antibodies in pigs,then compare the results with previous investigations on PCV4 seroepidemiology,so as to understand the prevalence and infection of PCV4 in swine populations more comprehensively.PCV4 Cap protein was successfully expressed in a prokaryotic expression system.By optimizing the reaction conditions,an indirect ELISA method based on PCV4 Cap protein was established.To investigate th...
