期刊论文

中文

畜牧兽医学报

0366-6964

2024

55

1

142-152

10.11843/j.issn.0366-6964.2024.01.015

32172758:国家自然科学基金 2021RC4060:湖南省科技厅科技创新团队项目 2022YFD1301101-1:国家重点研发计划 CARS-37:国家肉牛牦牛产业技术体系项目

本校为第一机构

旨在探究外源补充亮氨酸(Leu)对牛成肌细胞增殖的影响及其作用机理。本试验以牛成肌细胞为研究对象,利用CCK-8法和EdU染色法检测不同浓度的Leu对牛成肌细胞增殖的影响,确定Leu的最佳添加浓度;以最佳浓度Leu为处理...展开更多 旨在探究外源补充亮氨酸(Leu)对牛成肌细胞增殖的影响及其作用机理。本试验以牛成肌细胞为研究对象,利用CCK-8法和EdU染色法检测不同浓度的Leu对牛成肌细胞增殖的影响,确定Leu的最佳添加浓度;以最佳浓度Leu为处理组、0 mmol·L-1Leu为对照组进行转录组测序(RNA-Seq),筛选Leu调控牛成肌细胞增殖的关键通路。利用qRT-PCR验证关键通路中的关键调控基因,并通过添加抑制剂进行进...

This study was conducted to investigate the effect of exogenous leucine supplementation on the proliferation of bovine myogenic cells and its mechanism.The effects of different concentrations of Leu on the proliferation of bovine myoblasts were examined by CCK-8 and EdU...MORE This study was conducted to investigate the effect of exogenous leucine supplementation on the proliferation of bovine myogenic cells and its mechanism.The effects of different concentrations of Leu on the proliferation of bovine myoblasts were examined by CCK-8 and EdU s...