期刊论文

中文

畜牧兽医学报

0366-6964

2024

55

05

2267-2272

10.11843/j.issn.0366-6964.2024.05.044

2023NK2017:湖南省重点研发计划项目 202202AE090032:云南省重大科技专项计划 21A0125:湖南省教育厅重点项目 :湖南省普通高等学校科技创新团队支持计划

本校为第一机构

旨在建立一种可快速同时检测猪流行性腹泻病毒（porcine epidemic diarrhea virus, PEDV）、猪A群轮状病毒（porcine rotavirus type A, PoRVA）和猪丁型冠状病毒（porcine deltacoronavirus, PDCoV）三重荧光定量PCR方法。针对PEDV-N、PoRVA-VP6和PDCoV-M基因设计了引物和探针，条件优化后进行性能评估，并与商品化试剂盒检测对比。结果显示：本研究建立的三重RT-qPCR方法具有良好特异性，对PRRSV、PCV2和PRV等阳性核酸不发生扩增；具有较高的敏感性，PEDV、PoRVA和PDCoV的最低检测限均达1 copies·μL-1;重复性良好，组内和组间变异系数均小于1%;样本适应性广，检测不同样本类型时，变异系数均...

This paper aims to establish a triple fluorescence quantitative PCR method for the simultaneous detection of porcine epidemic diarrhea virus(PEDV),porcine rotavirus type A(PoRVA)and porcine deltacoronavirus(PDCoV).Primers and probes were designed for PEDV-N gene,PoRVA-VP6 gene and PDCoV-M gene,and their performance was evaluated after the conditions were optimized,and compared with commercial kits.The results showed that the triple RT-qPCR method established in this study had good specificity and did not amplify positive nucleic acids such as P...