National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21305039]; Foundation of Hunan ProvinceNatural Science Foundation of Hunan Province [14JJ3097]; Foundation of Hunan Agricultural University for 1515 Talents; Foundation of Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Resource Utilization; Foundation of Hunan Provincial Graduate Research and Innovation [CX2017B379]
机构署名:
本校为第一且通讯机构
院系归属:
理学院
生物科学技术学院
摘要:
A porous graphene (PG) bionanocomposite of PG, gold nanoparticles (AuNPs) and anti-indole-3-acetic acid (anti-IAA) antibody for sensitive and label-free amperometric immunoassay of IAA was reported. A PG film was produced by a pre-reduction/electrochemical reduction process on a glassy carbon electrode (GCE) and then a homogeneous AuNPs layer electrodeposition on the PG film. The anti-IAA antibody was immobilized onto the AuNPs through electrostatic adsorption and covalent conjugation. Scanning electron microscopy (SEM), transmission electron m...