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The DYW domain of RARE1 plays an indispensable role in regulating accD-C794 RNA editing in Arabidopsis thaliana

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成果类型:
期刊论文
作者:
Liu, Dan;Li, Zi-Ang;Li, Yi;Molloy, David P.;Huang, Chao
通讯作者:
Huang, C;Molloy, DP
作者机构:
[Huang, Chao; Li, Zi-Ang; Li, Yi; Liu, Dan] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China.
[Molloy, David P.] Chongqing Med Univ, Basic Med Coll, Dept Biochem & Mol Biol, Chongqing 400016, Peoples R China.
[Molloy, David P.] Chongqing Med Univ, Basic Med Coll, Ctr Mol Med & Canc Res, Chongqing 400016, Peoples R China.
通讯机构:
[Huang, C ] H
[Molloy, DP ] C
Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China.
Chongqing Med Univ, Basic Med Coll, Dept Biochem & Mol Biol, Chongqing 400016, Peoples R China.
语种:
英文
关键词:
Arabidopsis;AtECB2;Chloroplast RNA editing;RARE1;accD-C794
期刊:
Plant Science
ISSN:
0168-9452
年:
2023
卷:
334
页码:
111751
基金类别:
This work was supported by the National Natural Science Foundation of China (31900387), Natural Science Foundation of Hunan Province (2020JJ4037), the Research Foundation of Education Bureau of Hunan Province (2022B112) and The Chinese Government Ministry of Science and Technology (G2021035005L; DM).
机构署名:
本校为第一且通讯机构
院系归属:
生物科学技术学院
摘要:
The Arabidopsis pentatricopeptide repeat (PPR) proteins, required for accD RNA editing 1 (RARE1) and early chloroplast biogenesis 2 (AtECB2), each contain a DYW domain deemed essential for cytosine deamination at the accD-C794 RNA editing site in chloroplasts. Complementation assays using the rare1 mutant investigate the correlation between these PPRs and their respective DYW domain functions in RNA editing of accD-C794. The results demonstrate that the coding sequence of AtECB2 cannot replace that of RARE1. Moreover, rare1 mutants complemented...

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