Enhanced SDC-assisted digestion coupled with lipid chromatography-tandem mass spectrometry for shotgun analysis of membrane proteome

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成果类型：

期刊论文

作者：

Lin, Yong*;Wang, Kunbo;Liu, Zhonghua;Lin, Haiyan;Yu, Lijun

通讯作者：

Lin, Yong

作者机构：

[Liu, Zhonghua; Lin, Haiyan; Lin, Yong; Wang, Kunbo] Hunan Agr Univ, Coll Hort & Landscape, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Changsha 410128, Hunan, Peoples R China.

[Liu, Zhonghua; Yu, Lijun; Wang, Kunbo] Hunan Agr Univ, Coll Hort & Landscape, Minist Educ, Key Lab Tea Sci, Changsha 410128, Hunan, Peoples R China.

[Liu, Zhonghua; Lin, Yong] Hunan Agr Univ, Hunan Collaborat Innovat Ctr Utilizat Funct Ingre, Changsha 410128, Hunan, Peoples R China.

[Lin, Yong] Hunan Agr Univ, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Changsha 410128, Hunan, Peoples R China.

通讯机构：

[Lin, Yong] H

Hunan Agr Univ, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Changsha 410128, Hunan, Peoples R China.

语种：

英文

关键词：

Hydrophobic proteins;Integral membrane proteins;LC-MS/MS;Membrane proteome;SDC-assisted digestion;Shotgun analysis

期刊：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

ISSN：

1570-0232

年：

2015

卷：

1002

页码：

144-151

DOI：

10.1016/j.jchromb.2015.08.019

基金类别：

Planned Science and Technology Project of Hunan Province [201493131]; Science Research Foundation of Department of Education of Hunan Province [128064]; 1515 Talents Project of Hunan Agricultural University

机构署名：

本校为第一且通讯机构

院系归属：

园艺园林学院

摘要：

Despite the biological importance of membrane proteins, their analysis has lagged behind that of soluble proteins and still presents a great challenge mainly because of their highly hydrophobic nature and low abundance. Sodium deoxycholate (SDC)-assisted digestion strategy has been introduced in our previous papers, which cleverly circumvents many of the challenges in shotgun membrane proteomics. However, it is associated with significant sample loss due to the slightly weaker extraction/solubilization ability of 1% SDC. In this study, an enhan...

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Enhanced SDC-assisted digestion coupled with lipid chromatography-tandem mass spectrometry for shotgun analysis of membrane proteome

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