A new immunochromatographic assay for on-site detection of porcine epidemic diarrhea virus based on monoclonal antibodies prepared by using cell surface fluorescence immunosorbent assay

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成果类型：

期刊论文

作者：

Bian, Hongfen;Xu, Fei;Jia, Yumin;Wang, Lei;Deng, Shengchao;...

通讯作者：

Tang, Yong;Jia, Aiqing

作者机构：

[Tang, Yong; Wang, Lei; Bian, Hongfen; Xu, Fei] Jinan Univ, Dept Bioengn, Guangdong Prov Key Lab Mol Immunol & Antibody Eng, Guangzhou 510632, Guangdong, Peoples R China.

[Deng, Shengchao; Jia, Aiqing] Guangdong Haid Inst Anim Husb & Vet, Guangzhou 511400, Guangdong, Peoples R China.

[Jia, Yumin] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.

[Tang, Yong] Jinan Univ, Inst Food Safety & Nutr, Guangzhou 510632, Guangdong, Peoples R China.

通讯机构：

[Tang, Yong] J

[Jia, Aiqing] G

Jinan Univ, Dept Bioengn, Guangdong Prov Key Lab Mol Immunol & Antibody Eng, Guangzhou 510632, Guangdong, Peoples R China.

Guangdong Haid Inst Anim Husb & Vet, Guangzhou 511400, Guangdong, Peoples R China.

语种：

英文

关键词：

Porcine epidemic diarrhea virus;Cell surface fluorescence immunosorbent assay;Immunoassay;South of China;Swine stool analysis

期刊：

BMC Veterinary Research

ISSN：

1746-6148

年：

2019

卷：

期：

页码：

1-10

DOI：

10.1186/s12917-019-1773-4

基金类别：

This work was supported by the National key Research and Development Program of China (2016YFD0500600). The funders had no role in study design, or the collection, analysis, and interpretation of data. In addition, they were not involved in the writing of the report or the decision to submit the article for publication.

机构署名：

本校为其他机构

院系归属：

动物医学院

摘要：

Background: Porcine epidemic diarrhea virus (PEDV) is a highly effective pathogen that can cause death of new-born piglet, resulting in big economical loss in pig farming industry. For rapid detection of PEDV, a new immunochromatographic assay (ICA) based on monoclonal antibodies (mAbs) was developed in this study. Results: The mAbs were prepared by using PEDV positive hybridoma cells that were selected by using cell surface fluorescence immunosorbent assay (CSFIA). Fourteen mAbs against PEDV strain isolated from south of China were prepared. T...

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A new immunochromatographic assay for on-site detection of porcine epidemic diarrhea virus based on monoclonal antibodies prepared by using cell surface fluorescence immunosorbent assay

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