期刊论文

中文

生命科学研究

1007-7847

2001

5

3

275-276,276

10.16605/j.cnki.1007-7847.2001.03.017

湖南省生命科学联合中心重点资助项目 （ 1999-0 3）；

本校为第一机构

TE作为一种溶解DNA的常缓冲液,它对RAPD带型的影响往往没有受到重视.通过对TE缓冲液与RAPD带型之间关系进行了较系统的研究,结果说明TE对RAPD带型有明显影响;并且显示在Mg++为2 mmol/L时,TE是影响RAPD带型的敏感因素.还针对TE的不利影响,提出了有效的解决办法.

The relationship between TE buffer and Random Amplifed Polymorphic DNA (RAPD) profile has been studied and the result showed that TE buffer could change the profile of RAPD, especially at the 2 mmol/L of Mg ++ concentration. According to the results, TE was regarded as a sensitive factor changing RAPD profile. An alternative solu...