Plants are increasingly revealed to have the ability to shape microbiome composition and function by triggering rhizosphere metabolites. Rotation, a model of crop diversification, promotes crop production by influencing rhizosphere microbiome. However, the rhizosphere metabolites of different rice rotations are rarely reported and, in particular, the regulation of key metabolites on rhizosphere microbiome is unclear. To address this gap, we collected the bulk and rhizosphere soils of four crop rotations (rice-rice, tobacco-rice, rice-oilseed ra...