黄瓜绿斑驳花叶病毒(Cucumber green mottle mosaic virus,CGMMV)自1935年首次报道以来,已广泛分布于亚洲、欧洲、南美洲的多个国家和地区,对葫芦科作物的生产造成了严重损失。CGMMV主要有两种重要的传播途径,一是带毒种子造成远距离传播,二是授粉、嫁接等农事操作造成的田间传播。
摘要(英文):
Five leaf samples from watermelon plants with mosaic symptom in Shaoyang County were proved to be CGMMV-positive by RT-PCR detection using one pair of specific primers. The sequence of RT-PCR product (CGMMV-SY) was submitted to GenBank and showed identical t'o those of CGMMV coat protein genes in nu- cleotide databases after BLAST. The seeds of a rootstock variety widely used from Beijing were also detected CGMMV-positive and the RT-PCR product (CGMMV-JX) was of the same sequence as CGMMV-SY. They shared 100% nucleotide identities with som...