In this research, we set up eight temperature treatment with three repeats, which are 12.0℃, 13.5℃, 15.0℃, 16.5℃, 18.0℃, 19.5℃, 21.0℃ and 22.5℃, to determine the blood sugar level in blood serum of rainbow trout by using automatic biochemical analyzer (Model DS-2000) after one week treatment. The result showed that the blood sugar level in blood serum of rainbow trout keeps relatively stable under the temperature from 12.0℃ to 18.0℃, and increases rapidly from 18.0℃ to 19.5℃ varying by 63.72%, and reduces sharply from 19.5℃ to 22...