期刊论文

中文

基因组学与应用生物学

1674-568X

2012

31

2

160-166

10.3969/gab.031.000160

公益性行业（农业）科研专项（201003031） 湖南省科技厅项目（2010NK3021）共同资助 诚挚感谢湖南省常德市鼎城区植保站送检的疑似感染SRBSDV的水稻材料.

本校为第一机构

运用RT-PCR技术克隆了水稻南方黑条矮缩病毒(southern rice black-streaked dwarf virus,SRBSDV)湖南鼎城株系的基因组S10片段(SRBSDV-HuNDC S10),并对其全序列进行了测定和生物信息学分析.结果显示,SRBSDV-HuNDC S10片段全长为1 797 bp (登录号:JQ337964),含有1个ORF,编码557个氨基酸残基的衣壳蛋白,推测分子量约62.6 kD,推测等电点为7.62,与已报道的广东、海南和云南分离物病毒的S10作比较,它们的核苷酸相似性分别为99.7%、99.0%和98.4%,氨基酸相似性分别为100.0%、99.5%和99.3%.对SRBSDV-HuNDC S10及部分Fijiviruses病毒对应片段在5' URT与3' URT存在的保守序列和互补序列进行了归纳,对其ORF...

The full-length cDNA of the genome segment S10 of Dingcheng isolate of Southern Rice Black-Streaked Dwarf Virus was cloned and the complete nucleotide sequence was determined,as well as its bioinformatics analysis.Results show that the S10 is 1 797 bp（JQ337964）,has an open reading frame（ORF）,encodes a capsid protein containing 557 amino acid residues.Its molecular weight is about 62.6 kD and its isoelectric point is 7.62.Compared with other known viruses such as Guangdong isolates,Hainan isolates and Yunnan isolates,the nucleotide identitie...