期刊论文

Cloning and Expression Analysis of Tetrahydroberberine Oxidase (STOX) Gene in Macleaya cordata

中文

基因组学与应用生物学

1674-568X

2017

36

6

2458-2463

10.13417/j.gab.036.002458

31200615:国家自然科学基金 15KFXM09:国家重点实验室培育基地开放项目 2014SK2005:湖南省科技厅重点项目 13YJ09:湖南农业大学***项目

本校为第一机构

小檗碱是一种具有重要药用价值的次生代谢产物,目前该化合物主要来自于植物。四氢小檗碱氧化酶(STOX)是合成小檗碱的最后一步反应的酶,其在合成小檗碱的过程中发挥着关键的作用。本研究通过博落回转录组数据筛选到1个小檗碱氧化酶候选基因,并通过PCR技术对该序列进行克隆并进行生物信息学分析。经过克隆该序列中ORF区并用荧光定量PCR发现该基因的组织特异性表达规律与小檗碱代谢规律一致。该研究对下一步分析该基因功能、阐明博落回中STOX基因的合成机制和下一步运用植物代谢工程技术提高小檗碱在博落回中的含量具有重要意义。

Berberine is a kind of secondary metabolite that has important pharmaceutical value, which is mainly derived from plants at present. Tetrahydroberberine oxidase is the final enzyme in berberine biosynthesis and plays a key role in the synthesis process of berberine. The study screened a candidate STOX gene from M. cordata transcriptome database, and we cloned this sequence using PCR techniques and analyzed it by bioinformatics tools. We cloned the full length of its ORF region and found that the tissue specific expression pattern of the gene accorded with the metabolic regulation of berberine ...