It is of vital importance to establish a detection method for transgenic crops research. This study obtained the right flanking sequences of transgenic rice U41 by TAIL-PCR method, and revealed that the T-DNA insertion site was between 22 013^rd and 22 014^th in rice chromosome 1. Moreover, this study obtained the lett flanking sequences by designing primers, established an event-specific detection method for U41 transformation which could amplify a 529 bp specific band from 0.1% target genes, and set up a detection method with three primers which could identify the homozygous lines of U41 rap...
