期刊论文

Construction of Overexpression Vector and Genetic Transformation of Polyamine Transporter OsPUT1 Gene in Rice

中文

基因组学与应用生物学

1674-568X

2018

37

1

386-392

10.13417/j.gab.037.000386

湖南省研究生科研创新项目（CX2015B239）资助；

本校为第一机构

多胺(腐胺,亚精胺和精胺)不仅参与植物的生长发育调控,还与生物胁迫和非生物胁迫的抗性密切相关.由于多胺具有多重功能,细胞内多胺含量受到严格控制,例如受转运的调节.因此,在水稻中研究水稻多胺转运蛋白OsPUT1的功能至关重要.本研究以OsPUT1为研究对象,借助生物信息学分析,发现OsPUT1蛋白是一种疏水性蛋白,具有12个跨膜结构,定位于细胞核内.通过构建OsPUT1基因过表达载体,借助根癌农杆菌将目的基因转入到水稻愈伤组织的基因组中,以潮霉素进行筛选,通过PCR鉴定,成功获得了20株转基因植株.对其中的4株转基因水稻进一步作实时荧光定量PCR检测,显示全部转基因苗OsPUT1基因表达明显上调,实现了设计目...

Polyamine (putrescine, spermidine and spermine) is not only involved in the regulation of plant growth and development, but also closely related to the resistance to biotic and abiotic stresses. Because of its multiple functions, the intracellular polyamine content is strictly controlled, for example, regulated by transportation. Therefore, it is essential to study the function of polyamine transporter OsPUT1 in rice. In this study, we found that OsPUT1 is a hydrophobic protein with 12 transmembrane structures and is located in the nucleus via bioinformatics prediction. Through the constructio...