期刊论文

Cloning, Expression and Bioinformatics Analysis of Selenium-Binding Protein Gene OsSBP in Rice

中文

基因组学与应用生物学

1674-568X

2019

38

5

2110-2119

10.13417/j.gab.038.002110

湖南省自然科学基金(2018JJ2144) 湖南省银杏工程技术研究中心(2016TP2032) 湖南科技学院科学研究重点项目(17XKY005)共同资助.

本校为第一机构

为了探究水稻硒结合蛋白（selenium-binding protein, SBP）基因的功能,以水稻品种日本晴（Oryza sativa Japonica）为材料,采用同源克隆法获得Os SBP,并对其进行组织特异表达及生物信息学分析。结果表明,Os SBP基因cDNA序列为1 623 bp,包括长度为1 449 bp的CDS,碱基组成为A 23.3%、T 25.1%、G 28.9%、C 22.6%,编码482个氨基酸残基组成的蛋白质。Os SBP蛋白表现为弱酸性,且稳定、亲水;二级结构中以无规则卷曲和延伸链结构为主,三级结构同源建模成功,主要是螺旋和转角;亚细胞定位Os SBP主要分布在细胞质中,推测可能在运载结合、辅助因子中发挥重要作用,无跨膜结构域,无信号肽;该蛋白序列中存在24...

In order to explore the function of selenium-binding protein gene OsSBP in rice, this study took Oryza sativa Japonica as materials, obtained OsSBP by homology cloning strategy, and further analyzed with tissues expression and bioinformatics approaches. The results showed that the cDNA sequence of OsSBP was 1 623 bp length, encoding a protein of 482 amino acids. The CDS region of OsSBP was 1 449 bp length which compositions were 23.3% A, 25.1% T, 28.9% G and 22.6% C. The OsSBP protein was mildly acidic, stable, and hydrophilic, with random coil and extended strand as main components in seconda...