期刊论文

RNA-seq Transcriptome Analysis and Growth Related Gene Screening of Apalone spinifera

中文

基因组学与应用生物学

1674-568X

2019

38

4

1480-1487

10.13417/j.gab.038.001480

31372530:国家自然科学基金 31672640:国家自然科学基金 2016NK2115:湖南省科技计划

本校为第一机构

角鳖是一种具有极高经济价值的淡水动物,市场开发潜力较大。其生长上存在的显著差异,制约了角鳖养殖业的健康可持续发展。为了探索角鳖生长发育的相关分子机制,本研究以生长存在显著差异的快长组和慢长组角鳖肝脏为材料,采用Illumina HiSeq TM 2500高通量测序平台进行转录组分析,经过拼接组装共获得264 757 372条Clean reads,148 093个Unigene,序列平均长度为900.8 bp。将Unigene与NR、SWISSPROT、KOG、KEGG和GO进行比对,共有34 666条得到了注释结果。通过以快长组为对照组,慢长组为实验组进行基因表达情况对比,结果显示有2 246个基因表达存在差异,其中显著性上调的基因1 264个,显著性下调的基因...

Apalone spinifera is a kind of freshwater animal with high economic value,and has great potential for market development. The great differences in their growth restrict the healthy and sustainable development of Apalone spinifera breeding industry. In order to explore the molecular mechanism related to the growth in Apalone spinifera, we took the liver of Apalone spinifera in fast growth group and slow growth group with great differences as materials to analyze their transcriptions by using Illumina HiSeqTM 2500 high-throughput sequencing platform. In total 264 757 372 clean reads and 148 093 ...