期刊论文

Study on Expression Patterns of Gpat and Lpat Related to Oil Content in Rape under Different Fertilizer and Density Conditions

中文

基因组学与应用生物学

1674-568X

2019

38

2

714-720

10.13417/j.gab.038.000714

(湘财农指[2016]114号):油菜早熟品种选育和机械化生产技术研究示范 :作物种质创新与资源利用国家重点实验室培育基地开放课题“与脂肪酸合成相关基因表达量研究 :其功能验证”共同资助

本校为第一机构

油菜是我国第一大自产油料作物,具有重要的食用、饲用等价值。提高油菜籽含油量一直是育种家们不懈追求的目标之一,不同的栽培措施对油菜含油量有较大影响,为了研究不同施肥量、种植密度对油菜中控制含油量相关基因Gpat、Lpat表达量的影响,本研究采用qRT-PCR测定不同时期、不同肥密条件下Gpat、Lpat基因的表达量,并对其表达情况进行了分析。研究得知:Gpat、Lpat表达量变化规律是相同的,即:随着氮肥的增加而降低,随着密度的增加而升高,随着硼肥的增加而先升高后下降。此外,本研究在A1B3C1、A1B2C2及A2B3C2处理下可同时促进Gpat与Lpat的表达,是比较理想的肥密条件,可为提高油菜的含油量提供参考。...

Rape is the largest self-produced oil crop in China, and it has important food and feeding value. Raising oil content of rapeseed is always one of the goals pursued by breeders. The cultivation measures have great influence on the rapeseed oil content. In order to study the effect of different amount of fertilizer and planting density on the expression of oil content related gene Gpat and Lpat in Brassica napus, the expression of these genes was measured by qR T-PCR at different periods and different density and fertilizer conditions and it was then analyzed. The results showed that the change...