期刊论文

Optimization of Extraction and Alcohol Precipitation Process of Bletilla striata Polysaccharides Based on Response Surface Methodology

中文

基因组学与应用生物学

1674-568X

2019

38

8

3681-3689

10.13417/j.gab.038.003681

No.31671858:国家自然科学基金 No.31760084:国家自然科学基金 No.2015NK2039:湖南省重点项目 No.JDZ201701:林产化工工程湖南省重点实验室开放基金 No.2017sky04:国家级大学生创新创业训练计划项目

本校为第一机构

白及多糖的生物活性近来引起了人们的高度关注,本研究旨在对白及多糖的水提醇沉工艺进行系统的优化,为白及多糖的深入研究提供理论基础。在单因素试验的基础上,本实验采用基于BBD (Box-Benhnken Design)的响应面法,分别对回流提取工艺和醇沉工艺进行优化。对回流提取工艺的优化以回流提取率为评价指标,得到最优回流提取工艺为提取时间3 h,提取温度100℃,料液比例为1:34 (g:mL);对醇沉工艺的优化以醇沉分离率为评价指标,得到最优醇沉工艺为:乙醇浓度85%,醇胶比例8:1 (g:g),醇沉时间6 h;在此条件下,提取率达到26.44%。

Bletilla striata polysaccharide (BSP) has been attracting much attention due to its bioactivities recently, and this study was undertaken to optimize the water extraction and alcohol precipitation process of BSP, in order to provide a heoretical basis for the further study of Bletilla striata polysaccharide. On the basis of single factor experiment, the reflux extraction process and alcohol precipitation process were optimized by response surface methodology (RSM) based on Box-Behnken Design (BBD). Reflux extraction rate as evaluation criterion...