期刊论文

cDNA Cloning,Sequence Analysis and Expression Characterization of Insulin-like Growth Factors Ⅰ and Ⅱ in F_1 of Ctenopharyngodon idellus (♀)×Squaliobarbus curriculus (♂)

中文

基因组学与应用生物学

1674-568X

2020

39

04

1478-1489

10.13417/j.gab.039.001478

31802288:国家自然科学基金 31572615:国家自然科学基金 31272652:国家自然科学基金 2018T110833:中国博士后科学基金特别资助项目 2017M612560:中国博士后科学基金

本校为第一机构

为探究草鱼(♀)×赤眼鳟(♂)杂交F_1生长性状形成相关分子机理,本研究通过RACE技术(rapidamplification of cDNA ends,RACE)克隆了杂交F_1个体胰岛素样生长因子-Ⅰ(Insulin-like growth factors-Ⅰ,IGF-Ⅰ)和IGF-Ⅱ的cDNA全长序列,利用荧光定量PCR检测了两个基因的组织表达水平,并比较了杂交F_1生长性状大、小两个亚群的基因表达水平差异。结果显示:IGF-Ⅰ和IGF-Ⅱ的cDNA全长分别为824bp和1707bp。IGF-Ⅰ和IGF-Ⅱ蛋白均具有胰岛素样生长因子的典型特征,即包括信号肽和B、C、A、D和E 5个功能结构域,并具有6个保守的半胱氨酸。IGF-Ⅰ与IGF-Ⅱ的氨基酸序列同源性较低,仅为26%。IGF-Ⅰ与IGF-Ⅱ在杂交...

In order to investigate the molecule mechanism involved in growth trait formation of progeny from Ctenopharyngodon idellus (♀)×Squaliobarbus curriculus (♂),full-length cDNAs of Insulin-like growth factors-Ⅰ(IGF-Ⅰ) and IGF-Ⅱ were cloned by RACE technique.Tissue expression levels of the two genes were detected by qPCR,and the significance of expression level difference between the F_1 progeny of largest-subpopulation and smallest-subpopulation was compared.The results showed that full-length cDNAs of IGF-Ⅰ and IGF-Ⅱ were 824 bp and 1707 b...