期刊论文

Transcriptome Comparison and Immune Related Gene Analysis of Spleen of Chinese Soft-shelled Turtle Pelodiscus Sinensis

中文

基因组学与应用生物学

1674-568X

2020

39

12

5449-5456

10.13417/j.gab.039.005449

31672640:国家自然科学基金 2018YFD0900200:蓝色粮仓项目 18QN29:湖南农业大学科技项目 XCX18018:湖南农业大学大学生创新性实验计划

本校为第一机构

由于中华鳖集约化养殖的不断发展,导致其养殖环境不断恶化、疾病频发,严重制约了中华鳖养殖产业的可持续发展。为了揭示中华鳖抗病分子机制,本研究利用Illumina HiSeqTM2500高通量测序平台对感染嗜水气单胞菌的中华鳖脾脏组织进行转录组de novo测序,共获得48 884条Unigenes。将Unigene与COG、GO、KEGG、KOG、Pfam、Swissprot、egg NOG和NR数据库进行比对,共获得17 202条注释信息。生物信息学分析结果显示共有2 300条Unigenes表达差异显著,其中上调899条、下调1 401条。对差异基因进行KEGG通路富集分析,结果显示显著富集的前20个通路中有3条免疫相关通路（Intestinal immune network for Ig A pr...

With the development of intensive farming,the breeding environment is deteriorating and the disease is frequent.The sustainable development of the Chinese soft-shelled turtle breeding industry is considerably constrained.In order to explore the Chinese soft-shelled turtle response process against bacteria at the transcriptomic level,this study used Illumina HiSeqTM 2500 high-throughput sequencing technology to examine transcriptional profiles on Chinese soft-shelled turtle spleen.As a result,48 884 Unigenes were acquired after assembly and redundancy.Comparing Unigenes with COG,GO,KEGG,KOG,Pfa...