Isolation and Identification of an α-Galactosidase-Producing Lactosphaera pasteurii Strain and Its Enzymatic Expression Analysis

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成果类型：

期刊论文

作者：

Zhao, Yan;Zhou, Jinghui;Dai, Shan;Liu, Xiaozhu;Zhang, Xuewen

通讯作者：

Xuewen Zhang<&wdkj&>Xiaozhu Liu

作者机构：

[Zhang, Xuewen; Zhao, Yan] Key Lab Crop Epigenet Regulat & Dev Hunan Prov, Changsha 410128, Peoples R China.

[Zhang, Xuewen; Zhao, Yan; Dai, Shan] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China.

[Zhou, Jinghui] Hunan Agr Univ, Coll Hort, Changsha 410128, Peoples R China.

[Liu, Xiaozhu] Guizhou Inst Technol, Coll Food & Pharmaceut Engn, Guiyang 550000, Peoples R China.

通讯机构：

[Xuewen Zhang] K

[Xiaozhu Liu] A

Authors to whom correspondence should be addressed.<&wdkj&>College of Food & Pharmaceutical Engineering, Guizhou Institute of Technology, Guiyang 550000, China<&wdkj&>Key Laboratory of Crop Epigenetic Regulation and Development in Hunan Province, Changsha 410128, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China

语种：

英文

关键词：

alpha galactosidase;amino acid;edetic acid;fructose;galactose;galactoside;glucose;glycoside;lactose;oligosaccharide;raffinose;sodium chloride;sucrose;Carnobacteriaceae;chemistry;enzyme specificity;kinetics;metabolism;pH;soil;alpha-Galactosidase;Amino Acids;Carnobacteriaceae;Edetic Acid;Fructose;Galactose;Galactosides;Glucose;Glycosides;Hydrogen-Ion Concentration;Kinetics;Lactose;Oligosaccharides;Raffinose;Sodium Chloride;Soil;Substrate Specificity;Sucrose

期刊：

Molecules

ISSN：

1420-3049

年：

2022

卷：

期：

页码：

5942-

DOI：

10.3390/molecules27185942

基金类别：

This work was finished in Key Laboratory of Crop Epigenetic Regulation and Development in Hunan Province, and supported by the Science and Technology Project of Hunan Province (No. 2014FJ3082).

机构署名：

本校为其他机构

院系归属：

园艺园林学院

生物科学技术学院

摘要：

α-Galactosidase (EC 3.2.1.22) refers to a group of enzymes that hydrolyze oligosaccharides containing α-galactoside-banded glycosides, such as stachyose, raffinose, and verbascose. These enzymes also possess great potential for application in sugar production, and in the feed and pharmaceutical industries. In this study, a strain of Lactosphaera pasteurii (WHPC005) that produces α-galactosidase was identified from the soil of Western Hunan, China. It was determined that the optimal temperature and pH for this α-galactosidase were 45 °C and...

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Isolation and Identification of an α-Galactosidase-Producing Lactosphaera pasteurii Strain and Its Enzymatic Expression Analysis

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