期刊论文

Molecular Cloning and Expression Analysis of HGITM cDNA in Trionyx sinensis

中文

渔业科学进展

2095-9869

2019

40

6

173-179

10.19663/j.issn2095-9869.20180906001

31672640:国家自然科学基金 2017JJ3134:湖南省自然科学基金 2016NK2115:湖南省自然科学基金 17C0935:湖南省教育厅项目

本校为其他机构

为研究GHITM基因在中华鳖(Trionyx sinensis)胚胎发育及生长中的作用,本研究通过RT-PCR和RACE方法获得了中华鳖GHITM全长c DNA序列;采用实时荧光定量PCR对GHITM m RNA组织表达及不同温度孵化胚胎发育过程中的表达特性进行分析。结果显示,中华鳖GHITM c DNA序列长度为2650 bp,开放阅读框为1050 bp,5’非编码区为123 bp,3’非编码区为1477 bp,编码349个氨基酸,编码蛋白的等电点为10.01,分子量为37.12kDa。GHITM编码氨基酸序列由胞外区、跨膜区和胞内区组成,7个跨膜域组成跨膜区。GHITM编码氨基酸序列的同源性分析显示,中华鳖与锦龟(Chrysemys picta bellii)和绿海龟(Chelonia mydas)同属一个分支,...

In this study, we obtained the full-length cDNA of the GHITM gene from Trionyx sinensis for the first time using the RACE (rapid-amplification of cDNA ends) method. The full-length cDNA sequence was 2650 bp, including a 123 bp 5'-UTR, 1477 bp 3'-UTR, and 1050 bp open reading frame (ORF) that encoded 349 amino acid residues. The isoelectric point (pI) of this peptide was 10.01, and the molecular mass was 37.12 kDa. The amino acid sequence was composed of the extracellular region, the transmembrane region, and the intracellular region. The transmembrane region was composed of 7 transmembrane dom...