期刊论文

High-Throughput Sequencing Analysis of the Effects of Sanguinarine on Trionyx sinensis Intestinal Microbiota

中文

渔业科学进展

2095-9869

2021

42

01

177-185

10.19663/j.issn2095-9869.20191227001

国家自然科学基金项目（31672640）； 国家重点研究开发项目-蓝色粮仓（2018YFD0900200）共同资助～～；

本校为第一机构

为研究血根碱对中华鳖（Trionyx sinensis）肠道菌群多样性及丰度的影响,选取480只初始体重为（31.75±7.20） g的中华鳖,设置添加0的对照组（RS1）和添加50 （RS2）、100 （RS3）和150 mg/kg（RS4）的3个实验组。实验结束后,提取中华鳖中肠组织的总DNA,进行16S rRNA V3-4区基因组测序。结果显示,实验组的总操作分类单元（Operational taxonomic units, OTUs）数量显著高于对照组,肠道菌群复杂度及丰富度较对照组有明显增加。肠道菌群结构组成分析发现,RS1与RS2结构较为接近,而RS1与RS3、RS4较远。在门分类水平上,变形菌门（Proteobacteria）和衣原体门（Chlamydiae）是优势菌群;在属分类水平上...

To study the effect of sanguinarine on intestinal microbiota diversity in Trionyx sinensis, 480 individuals [initial weight (31.75±7.20) g] were randomly selected and divided into four groups. Three experimental diets RS2, RS3, and RS4 were formulated with 50, 100, and 150 mg/kg sanguinarine, respectively, and no sanguinarine was included in the control group. At the end of the experiment, the total DNA of the midgut tissue was extracted and the 16S rRNA V3-4 region was sequenced. The results showed that the total number of operational taxonom...