期刊论文

Xuefei Jiang

英文

Biochemical and Biophysical Research Communications

0006-291X

2022

614

85-91

10.1016/j.bbrc.2022.05.008

This research is Supported by Hainan Provincial Natural Science Foundation of China ( 2019RC309 ) and central public-interest scientific institution basal research fund for Chinese Academy of Tropical Agricultural Sciences ( 1630032019038 ). Conceived and designed the experiments, X.J.; Performed the experiments, Z.W.; Analyzed the data, Y.H., L.L. and Y.Z.; Contributed reagents/materials/analysis tools, Y.Z., Y.X. and F.Q.; Wrote the paper, Z.W. and Y.H. All authors have read and agreed to the published version of the manuscript.

本校为其他机构

A glycosyl transferase, isolated from Angelica decursiva a medical herb rich in coumarin, shows C-glycosyl transferase activity by in vitro activity assay using 5,7-dihydroxycoumarin as substrate, producing a C-glycosylated product at position C ' 8 along with the main product at C ' 6 position. Catalytic promiscuity assay shows that AdCGT also displays O- or C-glycosylation activity to other coumarins and flavonoids. When phloretin and 2,4,6-trihydroxyacetophenone were fed as substrates, AdCGT catalyzed the formation of di-C-glycosides. Therefore, AdCGT is a multifunctional glycosyltransferas...