期刊论文

中文

中国动物检疫

1005-944X

2024

41

03

5-9+47

10.3969/j.issn.1005-944X.2024.03.002

2023NK2017:湖南省重点研发项目

本校为第一机构

为了解湖南省2021-2023年无害化处理场猪瘟病毒(CSFV)、伪狂犬病病毒(PRV)、猪繁殖与呼吸综合征病毒(PRRSV)污染情况,采用RT-qPCR方法对采集的 1825份淋巴结、脾脏、耳尖等组织样品进行CSFV、PRV、PRRSV核酸检测,并对2022年衡南县PRRSV阳性样品进行分型检测及Nsp2基因测序.结果显示:CSFV、PRV、PRRSV的阳性检出率分别为3.01％、1.81％、30.14％;双重污染率为2.52％,以PRRSV与其他2种病原双重污染为主,三重污染率较低,为0.05％.2022年衡南县无害化处理场的PRRSV阳性检出率为24.12％,以春、冬季阳性率较高;北美型(NA-PRRSV)、高致病型(HP-PRRSV)、类NADC30毒株(NADC30-like)阳性检出率分别为98.39...

In order to investigate the contamination with classical swine fever virus(CSFV),pseudorabies virus(PRV)and porcine reproductive and respiratory syndrome virus(PRRSV)in bio-safety disposal plants in Hunan Province from 2021 to 2023,1 825 tissue samples including lymph nodes,spleens and ear tips were detected for nucleic acids of the three viruses by RT-qPCR,and positive samples of PRRSV from Hengnan County in 2022 were typed and sequenced for Nsp2 gene.The results showed that the positive detection rates of CSFV,PRV and PRRSV were 3.01％,1.81％...