期刊论文

中文

中国蔬菜

1000-6346

2008

9

10-14

国家科技基础性工作专项（2006FY11070902）；

本校为第一机构

根据GenBank植物EIN3基因家族的保守序列设计了1对引物,以纯雌性系、强雌性系、雌雄同株系、两性花株系的不同性别黄瓜总DNA为模板,经PCR扩增得到6个725 bp左右的基因片段并进行序列比较分析.结果表明,不同性型黄瓜肭基因编码区有14个SNPs,平均51 bp发现1个SNP,检出率为1.96%;其中有3个酶切位点突变,且发现编码区的SNPs有8个突变导致了氨基酸改变;初步建立了1个RFLP-Bfm I的酶切体系,发现在两性花株WI1983H中出现特异的酶切条带.

A pair of oligonucleotide primers was designed from conserved domain of EIN3 gene family. PCR amplifications were performed on different sex genotype cucumber DNA template and produced 6 fragments of 725 bp, respectively. After cloning and sequencing on CsEIN3 of different sex genotype cucumbers, BLAST and DNAMAN analysis found 14 single nucleotide polymorphisms （SNPs） in coding region, about one average 51 bp, selection rating 1.96 % , 3 mutation in enzyme digested site, eight SNPs mutation cause amino acid sequence variation. One set of sta...