期刊论文

Effect of Different Fertilization on Ammonia-oxidizing bacteria and Ammoniaoxidizing archaea in Red Soil Vegetable Field

中文

中国蔬菜

1000-6346

2015

7

33-39

10.3969/j.issn.1000-6346.2015.07.008

国家自然科学基金项目（31201688,31101413） 国家科技支撑计划项目（2012BAD151304） 中国博士后科学基金项目 （2012M511374） 湖南省科技厅一般项目（2012RS4036） 湖南农业大学“神农学者”专项

本校为第一机构

通过构建氨单加氧酶基因（amoA）克隆文库，研究在红壤蔬菜田上只施用磷钾化肥（PK）、只施氮磷钾化肥（NPK）、施用腐熟有机肥（DNPK）和施用新鲜有机肥（FNPK）等4种不同施肥处理的土壤氨氧化细菌（AOB）和氨氧化古菌（AOA）群落多样性及与土壤脲酶活性的相关性。结果表明：施加有机肥处理（DNPK和FNPK）的蔬菜田土壤的AOB文库和AOA文库OTU数量和Shannon指数高于只施用无机肥（NPK和PK）处理的蔬菜田土壤；DNPK和FNPK处理的土壤优势AOB菌群为多形亚硝化叶菌（Nitrosolobus multiformis），比例分别为88.5%和68.5%，NPK和PK处理的土壤优势AOB菌群为亚硝化单胞菌属（Nitrosospira sp．），比例分...

AdoptingamoA gene clone library construction method,this paper studied the diversity of ammonia-oxidizing bacteria(AOB) and ammonia-oxidizing archaea (AOA)and correlations between diversity index and urease activity in red soil vegetable field by different fertilization including decomposed organic fertilizer (DNPK),fresh organic fertilizer(FNPK),Nitrogen,Phosphorus and Potassium chemical fertilizer(NPK), Phosphorus and Potassium chemical fertilizer(PK).The results showed that OTU quantity,Shannon index of AOB library and AOA library represented organic fertilizer (DNPK and FNPK) were higher t...