期刊论文

中文

安徽农业科学

0517-6611

2008

36

6

2243-2244,2256

10.13989/j.cnki.0517-6611.2008.06.125

广东省科技攻关项目（2006B20101011）； 广东省自然科学基金项目（5011730）；

本校为第一机构

[目的]为尾叶桉资源开发及其转基因研究奠定基础。[方法]以尾叶桉无菌苗的下胚轴为外植体,研究不同激素种类及配比对其愈伤组织诱导、芽分化和增殖的影响,并分析不同浓度IBA对其生根的影响。[结果]不同激素组合对愈伤组织的诱导形成均有显著影响,诱导率为35.0%-95.0%。诱导尾叶桉愈伤组织形成的最适培养基为MS＋6-BA0.60 mg/L＋2,4-D0.10 mg/L,诱导芽分化的最适培养基为MS＋NAA0.10 mg/L＋TDZ2μmol/L,诱导芽增殖的最适培养基为MS＋6-BA0.40 mg/L＋NAA0.05 mg/L。尾叶桉再生苗的最适生根培养基为1/4 MS＋0.50 mg/LIBA。生根苗在自然环境中炼苗9 d后再移栽,其成活率在90%以上。[结论]接种初期暗培...

[Objective] The research aimed to lay the foundation for the resource development of Eucalyptus urophylla and its transgenic research.[Method] With hypocotyledonary axis in the aseptic seedling of E.urophylla as explants,the effects of different kinds of hormones and their matching on the callus induction,bud differentiation and proliferation were studied and the effects of different concentrations of IBA on its rooting were analyzed. [Result] Different hormone combinations all had significant effects on the callus formation with the induction ...