[Objective] To study the difference of hormone level and change rule and the difference of endogenous phytohormones in tobacco male sterile line and its maintainer line at growth stage. [Method] We selected three treatments of male sterile lines and maintiainer lines (K326,K394 and Yunyan 87) as the research materials,and adopted functional leaves and buds of seven different growth stages (seedling stage,rosette stage,prosperous growth for 10 d,squaring stage and central flower opening stage). Five endogenous hormone contents(IAA, GA,ABA,iPAs,ZRs) of sample from seven different growth stages w...