An efficient regeneration and transformation system for Poa pratensis L. was studied by using mature seeds of three species. An efficient induction and regeneration system from embryonic calli of these species was established. Mature seeds was used as the explant, wiped off part of endosperm and treated with concentrated hydrochloric acid; choose NB+ 2,4-- D 2mg/L ag induction culture medium, NB+ 6 -- BA 2mg/L as differential medium, 1/2NB+IAA 0.5mg/L+NAA 0. 5mg/L as radication medium) confirmed the concentrations of different antibiotics ...