期刊论文

中文

中国草地学报

1673-5021

2007

29

4

45-49

10.3969/j.issn.1673-5021.2007.04.008

湖南省自然科学基金项目“抗旱、抗除草剂目的基因转化草坪草获得转基因新材料的研究”（05JJ30036） 湖南省教育厅科学研究重点项目“转抗旱、抗除草剂双价基因冷季型草坪草新品系的培育”（05A022）资助

本校为第一机构

从外植体预处理方式、基本培养基、激素浓度等方面着手,对多年生黑麦草愈伤组织诱导、继代、不定芽分化及再生过程中的影响因素进行了研究,提高了再生频率.结果如下:切取胚端半粒种子,经75%乙醇处理lmin,0.1%HgCl处理15 min,在经无菌水清洗4～6次后接种方式较好;愈伤组织诱导培养基以MS+2,4-D 8.0 mg/L(或另添加甘露醇25 g/L)为佳;愈伤组织接种于MS+2,4-D 8.0mg/L+甘露醇25 g/L上进行1～2次继代后于NB+6-BA2.0 mg/L上进行分化;不定芽转接至MS+6-BA2.0 mg/L上进行扩繁或于1/2MS+NAA0.5mg/L+IAA0.5 mg/L上进行生根.

The factors influencing calli induction, subculture, differentiation and plant regeneration of Ryegrass which including explants pretreatment, basal medium and concentration of phytohormone were studied. The result showed that the half-seed to embryo, surface-sterilized by 0. 1% HgCl for 15 min after dipped in 75% ethanol for 1 min could be used as explants. The calli induced on medium of MS＋2, 4-D8.0 mg/L（with mannitol 25 g/L or not） were best. On medium of MS＋2,4-D8.0 mg/L＋ mannitol 25 g/L calli were subcultured for 1 to 2 times, then cu...