期刊论文

中文

中国草地学报

1673-5021

2018

40

3

14-19,48

10.16742/j.zgcdxb.2018-03-03

湖南省大学研究性学习与创新性实验计划项目（DFCXS201603） 湖南农业大学重大科研项目暨创新团队培育工程资助项目（17PYXM02）

本校为第一机构

本研究利用SCoT标记对洞庭湖地区9个野生南荻居群的遗传多样性和遗传结构进行分析。结果表明:27条SCoT引物共扩增出429条清晰的带,其中多态性带为394条,多态性条带比率为91.39%,平均每条引物扩增多态性条数是14.60条,多态性信息含量(PIC)为0.206~0.688,平均为0.394;该地区南荻遗传多样性丰富,遗传多样性指标水平较高(PPB=91.39%、H=0.3266、I=0.4911);其野生居群具有一定程度的遗传分化(G_(st)=0.1141),遗传变异主要存在于居群内;居群间的基因流(Nm)为3.88;根据非加权配对类平均法(UPGMA)聚类分析,将南荻9个野生居群分为三类,同一流域的居群聚为一类;材料间的遗传距离与地理距离不存在相关性。

Start codon targeted polymorphism (SCoT) was used to analysis nine M.lutarioriparius populations collected from Dongting Lake to reveal the genetic diversity and genetic structure in these populations.The results showed that:(1) 27 primers were selected from 36 SCoT primers,and a total of 429 distinct bands were amplified,of which 394 (91.39%) were polymorphic,the average was 14.60 per primer.The value of locus polymorphic information contents (PIC) ranged from 0.206 to 0.688,with an average of 0.394.(2)M.lutarioriparius in Dongting Lake showed...